Plant Biotechnology Partner

From gene to green, precisely.

Totinova designs and executes plant genetic transformation, genome editing, and tissue culture protocols for universities and biotechnology companies — turning research questions into regenerated, reproducible plants.

Start a project See our services
01
Design
Genetic Transformation
02
Edit
CRISPR / Cas Genome Editing
03
Culture
Tissue Culture & Micropropagation
04
Regenerate
Protocol Optimization
What we do

Laboratory work, partnership, and knowledge transfer

Nine core services organized around how research teams actually work with us — in the lab, on a project, or in a training room.

In the lab

Transformation, editing & regeneration

  • Plant genetic transformation
  • CRISPR/Cas genome editing
  • Plant tissue culture & micropropagation
  • Plant regeneration & protocol optimization
  • Custom transformation and regeneration protocols for recalcitrant species
On your project

R&D partnership & consulting

  • R&D project design and execution for universities and biotech companies
  • Scientific consulting and technology transfer in plant biotechnology
  • Research collaboration with universities, institutes & companies
In the room

Training & capability building

  • Specialized training programs in plant biotechnology
  • Hands-on laboratory workshops
Crops with history

Melon and tomato, two species we know well

Both crops carry centuries of cultivation history and, in the lab, real regeneration challenges. Two examples of where our protocols are put to work.

Melon grown as part of published transformation research
Add melon photo assets/photo-melon.jpg
M

Melon

Cucumis melo

Domesticated in Africa and Asia thousands of years ago and long prized across the Mediterranean and Middle East, melon remains genetically diverse and notoriously difficult to regenerate in vitro. We work on transformation and micropropagation protocols tuned to this variability, cultivar by cultivar.

Transformation Micropropagation Recalcitrant genotypes
Published protocol: Shirazi Parsa et al., 2023, Int. J. Mol. Sci. — doi.org/10.3390/ijms241311189
T

Tomato

Solanum lycopersicum

Brought from the Americas and transformed into a cornerstone of global agriculture, tomato is also the model species for plant genome editing. We use it for CRISPR/Cas construct validation, regeneration benchmarking, and applied trait research for partner programs.

CRISPR/Cas editing Regeneration Trait research
How we work

From first conversation to delivered protocol

A consistent process whether you're a university lab or an industry R&D team.

01 — Discovery

We scope the species and goal

A call to understand your species, existing protocols, timeline, and what success looks like.

02 — Proposal

We design the protocol

A written plan covering methodology, milestones, and expected outcomes before any work starts.

03 — Execution

We run the lab work

Transformation, editing, culture, or regeneration, with regular progress updates along the way.

04 — Delivery

We hand off results

Characterized plants, data, and documentation ready to publish, patent, or scale internally.

About Totinova

A precise, reliable partner for plant science

Totinova works at the intersection of genetic transformation, genome editing, and tissue culture, helping academic and industry teams move from a research question to a regenerated, characterized plant.

We take on the protocols that don't work off the shelf — recalcitrant species, low-efficiency regeneration, editing constructs that need tuning — and build the customized process your species and your timeline require.

Every engagement is built on scientific rigor, transparent methodology, and long-term reliability, for partners who need results they can publish, patent, or scale.

Peer-reviewed work

Shirazi Parsa, H., Sabet, M.S., Moieni, A., Shojaeiyan, A., Dogimont, C., Boualem, A., Bendahmane, A. (2023). CRISPR/Cas9-Mediated Cytosine Base Editing Using an Improved Transformation Procedure in Melon (Cucumis melo L.). International Journal of Molecular Sciences, 24(13), 11189.

doi.org/10.3390/ijms241311189 →
Innovation

Protocols built for your species

No generic pipelines — transformation and regeneration protocols are optimized for the plant in front of us, including recalcitrant and under-studied species.

Scientific excellence

Methodology first

Every project is designed with the same rigor expected of a peer-reviewed study, from construct design through data interpretation.

Reliability

A partner, not a vendor

Universities, institutes, and companies work with us across multiple projects because timelines and results hold up.

Sustainability

Plant science for the long term

Our work supports crop resilience and sustainable agriculture — research with consequences beyond the lab bench.

The Person Behind Totinova
Dr. Hadi Shirazi Parsa
Founder & Scientific Lead, Totinova

Hadi holds a PhD in Plant Molecular and Cellular Biology from Université Paris-Saclay and completed over four years of postdoctoral research at the Institute of Plant Sciences Paris-Saclay (IPS2). His expertise includes plant tissue culture, regeneration, genetic transformation, and CRISPR/Cas9 genome editing, with extensive experience in tomato and melon.

Totinova was founded to bring this expertise directly to research laboratories and biotechnology companies, with the founder personally involved in every project.

FAQ

Common questions

Most crop and model species, including recalcitrant ones. If a published protocol doesn't exist for your species, that's exactly the kind of project we take on.

Usually yes — seeds, explants, or tissue, depending on the species and protocol. We'll confirm exact requirements and any import/phytosanitary steps during the proposal stage.

It depends on the species and whether an existing protocol needs adapting or a new one needs developing — anywhere from a few weeks for optimization work to several months for a full transformation-to-regeneration pipeline on a recalcitrant species.

Yes. We work under NDA by default for industry partners, and agree on data and IP ownership terms with universities before any project begins.

Yes — our training and workshop services are built for exactly that, either alongside a project or as a standalone engagement in your own lab.

Get in touch

Let's talk about your species, your timeline, your goals

Whether it's a single custom protocol or an ongoing research partnership, tell us what you're working on.